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KMID : 1024420090130040326
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Food Engineering Progress
2009 Volume.13 No. 4 p.326 ~ p.334
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Purification of Antithrombotic Material from Auricularia auricular-judae Extracts and Its Antithrombotic Activity
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Park Young-Seo
Choi Hyuk-Joon
Choi Tae-Hyun
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Abstract
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Blood coagulation and aggregation of platelet are crucial events in the pathogenesis of various ischemic diseases. The substance which can prevent blood coagulation and platelet aggregation was extracted from wood ear mushroom (Auricularia auricular-judae) and its anticoagulation activity was investigated. The dried A. auricular-judae was extracted with 0.1 N NaOH and its supernatant was further extracted with methanol and ethanol followed by H2O. The resulting methanol soluble fraction showed significant antithrombotic activity in activated partial thromboplastin time, thrombin time, and prothrombin time assays with values of 100, 124, and 54 sec, respectively. This active substance was purified with DEAE-Sepharose CL6B and Sephacryl 400-HR and was found to be polysaccharide with the average molecular weight of over 150 kDa. This polysaccharide was xyloglucomannan of which the main component was mannose, and its anticoagulant activity was mostly mediated by inhibition of thrombin activity.
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KEYWORD
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Auricularia auricular-judae, anticoagulant activity, polysaccharide
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